Timur conquered large parts of Transoxiana (in modern day Central Asia) and Khorasan (parts of modern day Iran, Afghanistan, Uzbekistan, Tajikistan and Turkmenistan) from 1363 onwards with various alliances (Samarkand in 1366, and Balkh in 1369), and was recognized as ruler over them in 1370. Acting officially in the name of the Mongolian Chagatai ulus, he subjugated Transoxania and Khwarazm in the years that followed and began a campaign westwards in 1380. By 1389 he had removed the Kartids from Herat and advanced into mainland Persia from 1382 (capture of Isfahan in 1387, removal of the Muzaffarids from Shiraz in 1393, and expulsion of the Jalayirids from Baghdad). In 1394/95 he triumphed over the Golden Horde and enforced his sovereignty in the Caucasus, in 1398 subjugated Multan and Dipalpur in modern day Pakistan and in modern day India left Delhi in such ruin that it is said for two months "not a bird moved wing in the city". In 1400/01 conquered Aleppo, Damascus and eastern Anatolia, in 1401 destroyed Baghdad and in 1402 triumphed over the Ottomans at Ankara. In addition, he transformed Samarqand into the Center of the World. An estimated 17 million people may have died from his conquests.

After the end of the Timurid Empire in 1506, the Mughal Empire was later established in Afghanistan and India by Babur in 1526, who was a descendant of Timur through his father and possibly a descendant of Genghis Khan through his mother. The dynasty he established is commonly known as the Mughal Dynasty. By the 17th century, the Mughal Empire ruled most of India, but later declined during the 18th century. The Timurid Dynasty came to an end in 1857 after the Mughal Empire was dissolved by the British Empire and Bahadur Shah II was exiled to Burma.

Due to the fact that the Persian cities were desolated by previous wars, the seat of Persian culture was now in Samarkand and Herat. These cities became the center of the Timurid renaissance

Although the Timurids hailed from the Barlas tribe which was of Turkicized Mongol origin, they had embraced Persian culture, converted to Islam and resided in Turkestan and Khorasan. Thus, the Timurid era had a dual character, which reflected both the Turco-Mongol origins and the Persian literary, artistic, and courtly high culture of the dynasty.

During the Timurid era, Central Asian society was bifurcated and had divided the responsibilities of government and rule into military and civilian along ethnic lines. At least in the early stages, the military was almost exclusively Turko-Mongolian, and the civilian and administrative element was almost exclusively Persian. The spoken language shared by all the Turko-Mongolians throughout the area was Chaghatay Turkic. The political organization hearkened back to the steppe-nomadic system of patronage introduced by Genghis Khan. The major language of the period, however, was Persian, the native language of the Tājīk (Persian) component of society and the language of learning acquired by all literate and/or urban people. Already Timur was steeped in Persian culture and in most of the territories which he incorporated, Persian was the primary language of administration and literary culture. Thus the language of the settled "diwan" was Persian, and its scribes had to be thoroughly adept in Persian culture, whatever their ethnic origin. Persian became the official state language of the Timurid Empire and served as the language of administration, history, belles lettres, and poetry. The Chaghatay language was the native and "home language" of the Timurid family while Arabic served as the language par excellence of science, philosophy, theology and the religious sciences.

Persian literature, especially Persian poetry occupied a central place in the process of assimilation of the Timurid elite to the Perso-Islamic courtly culture. The Timurid sultans, especially Šāhrukh Mīrzā and his son Mohammad Taragai Oloğ Beg, patronized Persian culture. Among the most important literary works of the Timurid era is the Persian biography of Timur, known as "Zafarnāmeh" (Persian: ظفرنامه), written by Sharaf ud-Dīn Alī Yazdī, which itself is based on an older "Zafarnāmeh" by Nizām al-Dīn Shāmī, the official biographer of Timur during his lifetime. The most famous poet of the Timurid era was Nūr ud-Dīn Jāmī, the last great medieval Sufi mystic of Persia and one of the greatest in Persian poetry. In addition, some of the astronomical works of the Timurid sultan Ulugh Beg were written in Persian, although the bulk of it was published in Arabic. The Timurid ruler Baysunğur also commissioned a new edition of the Persian national epic Shāhnāmeh, known as Shāhnāmeh of Baysunğur, and wrote an introduction to it. According to T. Lenz

It can be viewed as a specific reaction in the wake of Timur's death in 807/1405 to the new cultural demands facing Shahhrokh and his sons, a Turkic military elite no longer deriving their power and influence solely from a charismatic steppe leader with a carefully cultivated linkage to Mongol aristocracy. Now centered in Khorasan, the ruling house regarded the increased assimilation and patronage of Persian culture as an integral component of efforts to secure the legitimacy and authority of the dynasty within the context of the Islamic Iranian monarchical tradition, and the Baysanghur Shahnameh, as much a precious object as it is a manuscript to be read, powerfully symbolizes the Timurid conception of their own place in that tradition. A valuable documentary source for Timurid decorative arts that have all but disappeared for the period, the manuscript still awaits a comprehensive monographic study.

In the realm of architecture, the Timurids drew on and developed many Seljuq traditions. Turquoise and blue tiles forming intricate linear and geometric patterns decorated the facades of buildings. Sometimes the interior was decorated similarly, with painting and stucco relief further enriching the effect. Timurid architecture is the pinnacle of Islamic art in Central Asia. Spectacular and stately edifices erected by Timur and his successors in Samarkand and Herat helped to disseminate the influence of the Ilkhanid school of art in India, thus giving rise to the celebrated Mughal (or Mongol) school of architecture. Timurid architecture started with the sanctuary of Ahmed Yasawi in present-day Kazakhstan and culminated in Timur's mausoleum Gur-e Amir in Samarkand. Timur's Gur-I Mir, the 14th-century mausoleum of the conqueror is covered with ‘’turquoise Persian tiles’’ Nearby, in the center of the ancient town, a Persian style Madrassa (religious school) and a Persian style Mosque by Ulugh Beg is observed. The mausoleum of Timurid princes, with their turquoise and blue-tiled domes remain among the most refined and exquisite Persian architecture. Axial symmetry is a characteristic of all major Timurid structures, notably the Shāh-e Zenda in Samarkand, the Musallah complex in Herat, and the mosque of Gowhar Shād in Mashhad. Double domes of various shapes abound, and the outsides are perfused with brilliantly colors. Timurs dominance of the region strengthened the influence of his capital and Persian architecture upon India.

In the realm of architecture, the Timurids drew on and developed many Seljuq traditions. Turquoise and blue tiles forming intricate linear and geometric patterns decorated the facades of buildings. Sometimes the interior was decorated similarly, with painting and stucco relief further enriching the effect. Timurid architecture is the pinnacle of Islamic art in Central Asia. Spectacular and stately edifices erected by Timur and his successors in Samarkand and Herat helped to disseminate the influence of the Ilkhanid school of art in India, thus giving rise to the celebrated Mughal (or Mongol) school of architecture. Timurid architecture started with the sanctuary of Ahmed Yasawi in present-day Kazakhstan and culminated in Timur's mausoleum Gur-e Amir in Samarkand. Timur's Gur-I Mir, the 14th-century mausoleum of the conqueror is covered with ‘’turquoise Persian tiles’’ Nearby, in the center of the ancient town, a Persian style Madrassa (religious school) and a Persian style Mosque by Ulugh Beg is observed. The mausoleum of Timurid princes, with their turquoise and blue-tiled domes remain among the most refined and exquisite Persian architecture. Axial symmetry is a characteristic of all major Timurid structures, notably the Shāh-e Zenda in Samarkand, the Musallah complex in Herat, and the mosque of Gowhar Shād in Mashhad. Double domes of various shapes abound, and the outsides are perfused with brilliantly colors. Timurs dominance of the region strengthened the influence of his capital and Persian architecture upon India.